International Conference on Molecular Biology and Medicine August 27-28, 2018 Dubai, UAE

Biography:

Zakaria Eltahir has completed his MSc in Cellular Pathology (Cardiff) and Certification of PG in Clinical Oncology (Birmingham) and PhD Histopathology at University of Wales UK. He has worked at Cardiff University, Swansea University as a Postdoctoral Scientist. He has then moved to London and worked at Royal Marsden NHS Foundation Trust and London University (ICR) as a Senior Clinical Researcher on Precision Medicine of the GI Cancers. He is a Registered Professional with HCPC in London as Clinical Scientist in Histopathology Pathology. He is currently working as an Associate Professor in Histopathology and Section Coordinator at Taibah University. He has 47 publications to his credit.

Abstract:

Gastroesophageal junction cancer or Barrett’s Adenocarcinoma (BA) is increasingly reported in the last four decades. In the Western world esophageal cancer reporting has shifted from SSC to AD. Evidence for some benefits from surveillance found could safe many patients’ lives at risk and relatively cost-effective. Clinical and scientific communities are faced by attempts to reduce mortality of BA as well as failure to detect most cases while at curable stage. It has been a real challenge to distinguish between patients who might develop cancer from those who wouldn’t. Indeed, diagnosis has been on debate and variable between different diagnostic schools, particularly for the LGD stage. Molecular markers are urgently needed to facilitate early tumorigenesis detection and to probably help in providing personalize cancer therapies. The ToGA (Trastuzumab for Gastric Cancer) project findings lead to the effective Trastuzumab (Herceptin) medicine to treat gastric and gastroesophageal patients and was approved by FDA in 2010. Her2-neu gene over expression assessment, after successfully used to eliminate those patients with mutated gene in breast cancer patients has provided a vital cure for about 25-30% of BA as in gastric cancer patients. Research in pathogenesis, biomarkers and clinical practice in precision medicine as future directions will be discussed.

Biography:

Aisan Asalipisheh has completed her Master’s degree from Islamic Azad University of Zanjan, Iran. She is a Microbiologist and Hygienist and Microbiology Lab Manager at Unilever Company. She has also worked as a Microbiologist in Mahram Company (sauce and dressing) and as a Microbiology Lab Manager at Gelatin Halal Company.

Abstract:

Introduction & Aim: Pseudomonas aeruginosa is the major cause of nosocomial infections and is considered as an opportunistic pathogen. Several reports indicate that the organism can also cause infections in healthy hosts. Further, evidence has suggested that there are no major differences in virulence between clinical and environmental isolates. Consuming raw vegetables that have been contaminated by this organism could have serious implications on human health. The aim of this study was to identify Pseudomonas aeruginosa virulence factor genes (Exotoxin A, Exoenzyme S, Elastase and Alginate genes) which are isolated from salads.

Method: In this cross-sectional study, 200 salad samples were collected from restaurants located in Qazvin during 6 months. After identification of isolates by biochemical tests, the antibiotic susceptibility test (Kirby-Bauer method) was done according to Clinical and Laboratory Standards Institute (CLSI) advice against 13 antibiotics. After DNA extraction from isolates, PCR with specific primers were done for detection of Exotoxin A, Exoenzyme S, Elastase and Alginate genes.

Results: Pseudomonas aeruginosa was isolated from 25 salad samples out of 200 samples (12.5%). The highest and lowest resistant against isolates (25 samples) related to Cefotaxime with 95% and Imipenem with 21.6% resistant respectively. Frequency of Exotoxin A, Exoenzyme S, Elastase and Alginate genes were 44%, 16%, 44% and 44%, respectively.

Conclusion: Our results showed that the frequency of Pseudomonas aeruginosa in salads was high. This study confirms the rapidity and sensitivity of PCR analysis in identifying isolates which contribute in early monitoring, accurate analysis and control of microbial risks in food products.

Biography:

Sheeba Parveen is a registered health care professional with the regulatory DHCC, Dubai. She holds a Master's degree in Molecular Microbiology from University of Karachi. She has pursued one year course on Medical Laboratory Training program exclusively in Molecular Pathology Department of Clinical Laboratory from well Known JCI, ISO and CAP accredited hospital in Pakistan. She has worked more than 11 years in the JCI, ISO and CAP accredited hospital as Molecular Specialist. She optimizes many assays including DNA sequencing, MLPA analysis for diagnosis of genetic disorders, methylation specific PCR for Prader-Willi/Angelman syndrome testing. She is also CAP Task Force Coordinator for the Molecular Pathology section of Aga Khan University Hospital, Karachi, Pakistan. She also had been involved in writing research papers and holding more than 9 local and international publications. Later, she joined Dow Diagnostic DHCC as a Molecular Specialist and served for almost 5 months and joined Pure Health as Molecular Biologist at Al Qassimi Hospital (MOH) in January 2018.

Abstract:

The largest known gene present in humans is located on the short arm of the X chromosome (at Xp21.2), and is called the Duchenne Muscular Dystrophy (DMD) gene. DMD gene has Open reading frame ~11.055 kb and it contains 79 exons MLPA (Multiplex Ligation-Dependent Probe Amplification) was developed as a general method to establish copy number of up to 45 nucleic acid sequences in one single reaction. This method has proved a reliable tool for the diagnosis of genetic diseases characterized by large gene deletions and duplications. This technique has made it possible to detect the entire dystrophin gene containing 79 exons in order to study the deletions and duplications in a fast and reliable manner. MLPA analysis was carried out using P034 and P035 probes purchased commercially from MRC, Holland (Amsterdam, Netherlands). The procedures were carried out according to the manufacturer’s recommendations. Briefly, 100 ng DNA was denatured and hybridized overnight at 60 °C with the SALSA probe mix 034 (DMD exons 1-10, 21-30, 41-50 and 61-70) and 035 (DMD exons 11-20, 31-40, 51-60 and 71-79). Samples were then treated with Ligase 65 for 15 min at 54 °C. The reactions were stopped by incubation at 98 °C for 5 minutes. IN THE LAST Step PCR amplification was done using SALSA FAM PCR primers, and after amplification amplified products are run on Genetic analyzer ABI Prism 3100 (Applied Bio systems, USA) with the following modules: Capillaries 36 cm, Polymer POP-4, run temperature 60 °C, capillary fill volume 184 steps, pre-run voltage 15 kV, pre-run time 180 seconds, injection voltage 3.0 kV, injection time 10-30 seconds, run voltage 15 kV, data delay time 1 second and run time 1500 seconds.

Biography:

Seyed Massoud Houshmand has completed his PhD in Medical Molecular Genetic from Gothenburg University, Gothenburg, Sweden. He is the Head of the Genetic Diagnostic Laboratory, Faculty Member of National Institute for Genetic Engineering and Biotechnology and  Responsible Director of Personalized Medicine journal. He has organized about 22 workshops and seminars and has published more than 220 papers and 17 books. He is the Winner of Best Iranian Researcher in Medical Genetic 2010, Winner of ISESCO prizes in Science & Technology 2014 and winner of Best Iranian Researcher 2015.

Abstract:

A genetic consultation is a health service that provides information and support to people who have or may be at risk for genetic disorders. In genetic counseling, the professional’s help people learn about genetic conditions, find out their chances of being affected by having a child or another genetic conditioner and make informed decisions about testing and treatment. In other words, the professional investigates the problem present in the family, interprets information about the disorder, analyzes the inheritance patterns and recurrence risk, and reviews available options with the family. This workshop will discuss a variety of topics such as:

Draw pedigree for Mendelian and non Mendelian disorders with Example for better understanding of:

1. Metabolic disorders
   1. Micromulecular Disorders (PKU)
   2. Macromulecular Disorders (MPS)
2. Neuromuscular disorders
   1. Autosomal Dominant (CMT)
   2. Autosomal Receive (SMA)
   3. X-linked (DMD)
   4. 3 nucleotide Repeats (HD)
   5. Mitochondrial Disorders (LHON)
3. Immunodeficiency disease.
   1. Autosomal Dominant (CGD)
   2. Autosomal Receive (SCID)
   3. X-linked (Wiscot-Aldrich)

Biography:

Reza Saghiri is an Associate Professor working in Pasteur Institute of Iran, Tehran. His research interest is about proteins, enzymes, isoenzymes, trace elements in diseases. He has published more than 35 papers in PubMed and other journals.

Abstract:

Introduction: Gaucher Disease (GD) is the most frequent autosomal recessive disorder of Lysosomal Storage Disease (LSDs). In this study, we set up the GD diagnosis standard fluorimetric assay.

Materials & Methods: We investigated the spectrum of mutations in GBA gene in 33 Gaucher patients from different Iranian populations by DNA sequencing, ARMS technique and PCR-RFLP, the GBA and CT enzyme activity in GD patients, 47 relative carriers and 105 age and sex matched healthy individuals was set up. Additionally, patients and controls were cultured in RPMI medium. The cell culture supernatant and plasma concentrations of IL3 and IL11 were determined by ELISA.

Results: For GBA activity, we determined the cut-off level of 1.61µmol/h/lit. The GBA activity test sensitivity and specificity were 96.9% and 100% respectively. Using cut-off level of 80.75% nmol/ml/h, sensitivity and specificity and CT activity were 93.9% and 100% respectively. Both GBA and CT activities of relative carriers were significantly different compared to GD patients and controls; however, their enzyme activities had an overlap (p<0.001). Compared to treated GD patients, untreated one had a significant higher CT activity (p=0.021). Chitotriosidase activity had a positive correlation with age in normal group (p=0.006; r=0.314. In GD patients, there was a direct correlation between TAC and hemoglobin concentration (p=0.035; r=0.369).

Conclusion: We designed and suggest rapid method (PCR-RFLP) for the first two incident mutations and designed an ARMS-PCR technique for primitive molecular screening of GD patients. These findings could be useful in screening programs and understanding of the molecular basis of GD.

Biography:

Mohammad Shabani is an Associate Professor at the Iran University of Medical Sciences (IUMS). He has received his PhD in Clinical Biochemistry from the University of Akron. USA in 1996. He has been the Head of Biochemistry Department at IUMS since 2009. His research was in drug design and delivery systems involving wound healing, NO metabolism, non-invasive laser imaging system and cancer research. US patent “Polymeric Wound healing Accelerators”1996. He has participated and presented articles in many national and international conferences and published more than 40 articles in different journals.

Abstract:

Introduction & Aim: Diabetic wounds have been the area of challenge since many years using different approaches to improve the problem. Although, application of laser on wound healing have already been examined, this study was designed to investigate the efficacy of a combination of 670 nm and 810 nm lasers irradiation for possible immune-stimulation of macrophage-derived NO for healing of diabetic and control wounded rat model.

Method: 36 Sprague-Dawley male rats were used and divided into four groups: DML (10), DMNL (10), CL (8) and CNL (8). Diabetes was induced by IP injection of 55 mg/kg of streptozotocin. A full-thickness circular wound was made on the back of all rats. Rats were transferred to metabolic cages for 24 hours urine collection. Rats were selected to be irradiated directly upon their wound with a combination of 670 nm (100 mw, 2 J/cm²) and 810 nm (50 mw, 1 J/cm²) every other day. Wound imaging (VIA) were performed on days 0, 7, 12, 16, 20 and 22. NO was measured by NO analyzer as biochemical index for healing process. P<0.05 was assigned as significant differences between groups.

Result: Percent Open Wound Area (POWA ‰) was significantly lower in the Diabetic Laser group in comparison to the Diabetic Non-Laser group in all measurement days. Also the POWA decreased between DML and DMNL groups on post-wounding as compared to control groups (P=0.021; mean difference=19.7%). NO production was increased upon wounding in DML group as compared to DMNL group. In addition, NO production increased dramatically in CL group as compared to CNL (P<0.05).

Conclusion: Our study showed that the irradiation of diabetic wounds with a combination of low dose 670 nm and 810 nm lasers accelerates wound healing process by NO production using photo-stimulation (LLL) technique of which would be new approach to wound healing.

Biography:

Lihini Ranesha Weerakkody has obtained Bachelor of Veterinary Science from University of Peradeniya, Sri Lanka. She has completed the Veterinary Internship and has worked as an Assistant Lecturer at University of Peradeniya. Currently, she is a Postgraduate student at the Faculty of Medicine, University of Colombo, Sri Lanka, following the Master of Science in Biochemistry and Molecular Biology. She is a Registered Veterinary Surgeon at the Veterinary Council of Sri Lanka since 2017.

Abstract:

Currently there are several protocols to extract bacterial genomic DNA based on different principles. However, most of these methods use hazardous organic solvents, including phenol and chloroform, whereas certain protocols use expensive enzymes including RNases and proteinases. This study was designed to introduce a simple, rapid, non-toxic, inexpensive and effective genomic DNA isolation procedure without using toxic chemicals and costly enzymes for Caldimonas manganoxidans; a Gram negative bacteria. Initially the bacterial cell pellet was re-suspended in TEN (Tris-HCl, EDTA, NaCl) and TENST buffer (Tris-HCl, EDTA, NaCl, SDS, Triton-X-100) and incubated. After that, saturated NaCl was added and centrifuged for protein precipitation. Then the supernatant was transferred and absolute ethanol was added. Finally, the tubes were centrifuged to precipitate genomic DNA. A modified salting-out method was also used to extract DNA from the same organism. The two methods were compared in terms of the quality and the quantity of DNA. The DNA yield was assessed by gel electrophoresis and the quality of DNA was verified by Polymerase Chain Reaction (PCR). The novel method showed better results with regard to the DNA yield and the quality of DNA. The novel method was less time-consuming compared to the other method. No inhibition was observed in subsequent PCR amplifications. The presented method is rapid, inexpensive and useful for routine DNA isolation from Gram negative bacteria such as Caldimonas. Quality and repeatability of the novel method were found to be adequate for subsequent molecular applications.