Day 2 :
Keynote Forum
Seyed Massoud Houshmand
National Institute for Genetic Engineering and Biotechnology, Iran
Keynote: Usage of P4 medicine
Time : 10:00-11:00
Biography:
Seyed Massoud Houshmand has completed his PhD in Medical Molecular Genetic from Gothenburg University, Gothenburg, Sweden. He is the Head of the Genetic Diagnostic Laboratory, Faculty Member of National Institute for Genetic Engineering and Biotechnology and Responsible Director of Personalized Medicine journal. He has organized about 22 workshops and seminars and has published more than 220 papers and 17 books. He is the Winner of Best Iranian Researcher in Medical Genetic 2010, Winner of ISESCO prizes in Science & Technology 2014 and winner of Best Iranian Researcher 2015.
Abstract:
Medicine can move from a reactive to a proactive discipline over future decade—a discipline that's predictive, personalized, preventive and participatory (P4). P4 medicine is fueled by systems approaches to sickness, rising technologies and analytical tools. Recent advances in genetics have led to the fast and comparatively cheap assortment of patient molecular information as well as multiple kinds of omics information. The combination of this information with clinical measurements has the potential to impact on our understanding of the molecular basis of illness and on disease management.
a) The use of combined data (genetics, or otherwise) about a person to predict treatment response and thereby to help improve his health.
b) The use of combined information (genetics, or otherwise) about a person to predict illness prognosis or treatment response and thereby improve that person’s health.
c) The use of combined data (genetics, or otherwise) about a person to predict illness condition, illness prognosis or treatment response and thereby improve that person’s health
Some of the foremost vital advantages include: 1) Higher medication choice. 2) Safer dosing choices. 3) Enhancements in drug development.
Systems medicine is an approach to understand illness through an integration of huge patient datasets. It offers the chance for personalized ways for healthcare through the improvement of a brand new taxonomy of illness. The application of P4 medicine will be discussed in this study.
- Molecular Biology, Microbiology & Pathology | Molecular Toxicology, Pharmacology & Drug Designing | Biochemistry, Metabolomics & Molecular Medicine | Medical Diagnosis, Microbiology & Molecular Genetics
Location: Dubai, UAE
Chair
Seyed Massoud Houshmand
National Institute for Genetic Engineering and Biotechnology, Iran
Co-Chair
Zakaria Eltahir
Taibah University, Saudi Arabia
Session Introduction
Reza Saghiri
Pasteur Institute of Iran, Iran
Title: The survey of mutation (GBA) gene and enzyme chitotriosidase in Gaucher disease
Time : 11:15-11:45
Biography:
Reza Saghiri is an Associate Professor working in Pasteur Institute of Iran, Tehran. His research interest is about proteins, enzymes, isoenzymes, trace elements in diseases. He has published more than 35 papers in PubMed and other journals.
Abstract:
Introduction: Gaucher Disease (GD) is the most frequent autosomal recessive disorder of Lysosomal Storage Disease (LSDs). In this study, we set up the GD diagnosis standard fluorimetric assay.
Materials & Methods: We investigated the spectrum of mutations in GBA gene in 33 Gaucher patients from different Iranian populations by DNA sequencing, ARMS technique and PCR-RFLP, the GBA and CT enzyme activity in GD patients, 47 relative carriers and 105 age and sex matched healthy individuals was set up. Additionally, patients and controls were cultured in RPMI medium. The cell culture supernatant and plasma concentrations of IL3 and IL11 were determined by ELISA.
Results: For GBA activity, we determined the cut-off level of 1.61µmol/h/lit. The GBA activity test sensitivity and specificity were 96.9% and 100% respectively. Using cut-off level of 80.75% nmol/ml/h, sensitivity and specificity and CT activity were 93.9% and 100% respectively. Both GBA and CT activities of relative carriers were significantly different compared to GD patients and controls; however, their enzyme activities had an overlap (p<0.001). Compared to treated GD patients, untreated one had a significant higher CT activity (p=0.021). Chitotriosidase activity had a positive correlation with age in normal group (p=0.006; r=0.314. In GD patients, there was a direct correlation between TAC and hemoglobin concentration (p=0.035; r=0.369).
Conclusion: We designed and suggest rapid method (PCR-RFLP) for the first two incident mutations and designed an ARMS-PCR technique for primitive molecular screening of GD patients. These findings could be useful in screening programs and understanding of the molecular basis of GD.
Mohammad Shabani
Iran University of Medical Sciences, Iran
Title: The effects of macrophage-derived Nitric Oxide (NO) on healing diabetic and non-diabetic wounded rat model using Low Level Laser (LLL)
Time : 11:45-12:15
Biography:
Mohammad Shabani is an Associate Professor at the Iran University of Medical Sciences (IUMS). He has received his PhD in Clinical Biochemistry from the University of Akron. USA in 1996. He has been the Head of Biochemistry Department at IUMS since 2009. His research was in drug design and delivery systems involving wound healing, NO metabolism, non-invasive laser imaging system and cancer research. US patent “Polymeric Wound healing Accelerators”1996. He has participated and presented articles in many national and international conferences and published more than 40 articles in different journals.
Abstract:
Introduction & Aim: Diabetic wounds have been the area of challenge since many years using different approaches to improve the problem. Although, application of laser on wound healing have already been examined, this study was designed to investigate the efficacy of a combination of 670 nm and 810 nm lasers irradiation for possible immune-stimulation of macrophage-derived NO for healing of diabetic and control wounded rat model.
Method: 36 Sprague-Dawley male rats were used and divided into four groups: DML (10), DMNL (10), CL (8) and CNL (8). Diabetes was induced by IP injection of 55 mg/kg of streptozotocin. A full-thickness circular wound was made on the back of all rats. Rats were transferred to metabolic cages for 24 hours urine collection. Rats were selected to be irradiated directly upon their wound with a combination of 670 nm (100 mw, 2 J/cm2) and 810 nm (50 mw, 1 J/cm2) every other day. Wound imaging (VIA) were performed on days 0, 7, 12, 16, 20 and 22. NO was measured by NO analyzer as biochemical index for healing process. P<0.05 was assigned as significant differences between groups.
Result: Percent Open Wound Area (POWA ‰) was significantly lower in the Diabetic Laser group in comparison to the Diabetic Non-Laser group in all measurement days. Also the POWA decreased between DML and DMNL groups on post-wounding as compared to control groups (P=0.021; mean difference=19.7%). NO production was increased upon wounding in DML group as compared to DMNL group. In addition, NO production increased dramatically in CL group as compared to CNL (P<0.05).
Conclusion: Our study showed that the irradiation of diabetic wounds with a combination of low dose 670 nm and 810 nm lasers accelerates wound healing process by NO production using photo-stimulation (LLL) technique of which would be new approach to wound healing.
Biography:
Lihini Ranesha Weerakkody has obtained Bachelor of Veterinary Science from University of Peradeniya, Sri Lanka. She has completed the Veterinary Internship and has worked as an Assistant Lecturer at University of Peradeniya. Currently, she is a Postgraduate student at the Faculty of Medicine, University of Colombo, Sri Lanka, following the Master of Science in Biochemistry and Molecular Biology. She is a Registered Veterinary Surgeon at the Veterinary Council of Sri Lanka since 2017.
Abstract:
Currently there are several protocols to extract bacterial genomic DNA based on different principles. However, most of these methods use hazardous organic solvents, including phenol and chloroform, whereas certain protocols use expensive enzymes including RNases and proteinases. This study was designed to introduce a simple, rapid, non-toxic, inexpensive and effective genomic DNA isolation procedure without using toxic chemicals and costly enzymes for Caldimonas manganoxidans; a Gram negative bacteria. Initially the bacterial cell pellet was re-suspended in TEN (Tris-HCl, EDTA, NaCl) and TENST buffer (Tris-HCl, EDTA, NaCl, SDS, Triton-X-100) and incubated. After that, saturated NaCl was added and centrifuged for protein precipitation. Then the supernatant was transferred and absolute ethanol was added. Finally, the tubes were centrifuged to precipitate genomic DNA. A modified salting-out method was also used to extract DNA from the same organism. The two methods were compared in terms of the quality and the quantity of DNA. The DNA yield was assessed by gel electrophoresis and the quality of DNA was verified by Polymerase Chain Reaction (PCR). The novel method showed better results with regard to the DNA yield and the quality of DNA. The novel method was less time-consuming compared to the other method. No inhibition was observed in subsequent PCR ampliï¬cations. The presented method is rapid, inexpensive and useful for routine DNA isolation from Gram negative bacteria such as Caldimonas. Quality and repeatability of the novel method were found to be adequate for subsequent molecular applications.